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. 2019 Nov 21;9:17312. doi: 10.1038/s41598-019-53611-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RNA expression in CRISPRi mouse lines. (a) Quantitative RT-qPCR analysis of transgene mRNA in L5 vertebrae from 5-week-old mice of line 157 WT (n = 6) and TG (n = 6), line 167 WT (n = 4) and TG (n = 5), and line 178 WT (n = 6) and TG (n = 3). Both sexes were included. Transgenic compared to WT littermates of the same line using Student’s t test. (b) Quantitative RT-qPCR analysis of Tnfsf11 mRNA in L5 vertebrae from 5-week-old mice of line 157 WT (n = 6) and TG (n = 6), line 167 WT (n = 4) and TG (n = 5), and line 178 WT (n = 6) and TG (n = 3). Both sexes were included. Transgenic compared to WT littermates of the same line using Student’s t test. (c) Correlation analysis of the values shown in a and b. Correlation significance and line of best fit included. (d) Quantitative RT-qPCR analysis of transgene mRNA in thymus from 5-week-old mice of line 157 WT (n = 6) and TG (n = 7), line 167 WT (n = 4) and TG (n = 5), and line 178 WT (n = 5) and TG (n = 3). Both sexes were included Transgenics compared to WT littermates of the same line using Student’s t test. (e) Quantitative RT-qPCR analysis of Tnfsf11 mRNA in thymus from 5-week-old mice of line 157 WT (n = 6) and TG (n = 7), line 167 WT (n = 4) and TG (n = 5), and line 178 WT (n = 5) and TG (n = 3). Both sexes were included. Transgenic compared to WT littermates of the same line using Student’s t test. (f) Correlation analysis of the values shown in (d,e). Correlation significance and line of best fit included.