Fig. 3.

Dynamic coupling of [Ca²⁺]_mito to [Ca²⁺]_cyto transients in neurons in vivo. a Schematic showing [Ca²⁺]_cyto transients with or without coupled [Ca²⁺]_mito transients. N_cyto or N_mito: number of cytosolic or mitochondrial Ca²⁺ transients observed. Coupling fidelity is defined as N_mito/N_cyto × 100%. b Averaged [Ca²⁺]_cyto transients in dendrites (upper panel) and somas (lower panel). 10–29 traces were used for each group. c, d Correlation of amplitudes of [Ca²⁺]_cyto and [Ca²⁺]_mito transients for coupled events in dendrites (c) and somas (d). (c: n = 23 paired events from 6 mice; d: n = 17 paired events from 8 mice). e Coupling fidelity in dendrites and somas (n = 47 from 6 mice in dendrites, n = 23 from 8 mice in soma). Data are presented as mean ± SEM. *P < 0.05. **P < 0.01. ***P < 0.0001, unpaired T-test. n.s.: no significance. Source data are provided as a Source Data file.