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. 2019;10(5-6):119–133. doi: 10.18632/genesandcancer.197

Figure 2. HDAC8 regulates MGMT protein levels.

Figure 2

A. Extracts from T98G cells treated with 15, 20 and 30 µM PCI34051 for 24 hours were subject to Western blotting with MGMT, tubulin, γH2AX and H3 antibodies. B. Extracts from T98G cells expressing stable shHDAC8 (1- TRCN0000350469, 2-TRCN0000004852 and 3-TRCN0000314874) and treated with TMZ for 48 hours were subject to Western blotting with MGMT, tubulin and HDAC8 antibodies. C. Quantitative RT-PCR analysis of mRNA from T98G cells expressing stable shHDAC8 (TRCN0000350469). D. Extracts from T98G and U87 cells expressing stable FLAG-MGMT and treated with PCI34051 for 24 hours were subject to Western blotting with FLAG and tubulin antibodies. E. Extracts from U87 cells were subject to Western blotting with MGMT, vinculin and HA antibodies.