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. 2019;10(5-6):119–133. doi: 10.18632/genesandcancer.197

Figure 4. ADRM1 regulates MGMT protein levels through HDAC8.

Figure 4

A. Extracts from T98G cells expressing shADRM1 (1-TRCN0000286432, 2-TRCN0000293817) were subject to Western blotting with MGMT, actin, tubulin and ADRM1 antibodies. B. Quantitative RT-PCR analysis of mRNA from T98G cells stably expressing shADRM1 (TRCN0000286432). C. Extracts from T98G cells expressing FLAG-MGMT, shHDAC8 (TRCN0000350469) and shADRM1 (TRCN0000286432) were subject to Western blotting with FLAG and tubulin antibodies. D. Extracts from T98G cells either expressing HA-ADRM1, FLAG-HDAC8 or treated with 15 µM PCI34051 for 24 hours were subject to Western blotting with MGMT, HA, FLAG, PSMD1 and tubulin antibodies.