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. 2019;10(5-6):119–133. doi: 10.18632/genesandcancer.197

Figure 5. HDAC8 inhibition induces DNA damage.

Figure 5

A. Extracts from U87 cells treated with PCI34051 for 24 hours were subject to Western blotting with γH2AX and H3 antibodies. B. Extracts from T98G cells treated with 15 µM PCI34051 for 24 hours were subject to Western blotting with phosphorylated ATM S1981, ATM, vinculin, γH2AX and H3 antibodies. C. Immunofluorescence demonstrating γH2AX expression in U87 cells treated with TMZ and 15 µM PCI34051 for 24 hours. D. Immunofluorescence showing 53BP1 and γH2AX expression in T98G cells treated with PCI34051 for 24 hours. E. Alkaline comet assay analysis of T98G cells treated with 20, 30 and 40 µM PCI34051 for 24 hours, n > 20, ***p < 0.0005. F. Extracts from U87 cells expressing shHDAC8 (1- TRCN0000350469, 2-TRCN0000004852) and treated with TMZ for 48 hours were subject to Western blotting with γH2AX, H3, HDAC8 and tubulin antibodies. G. Extracts of U87 cells expressing FLAG-HDAC8 and treated with TMZ for 48 hours were subject to Western blotting with γH2AX and H3 antibodies.