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. 2019;10(5-6):119–133. doi: 10.18632/genesandcancer.197

Figure 6. HDAC8 inhibition induces cell cycle arrest.

Figure 6

A. Flow cytometry analysis of T98G cells treated with 15 µM PCI34051 for 24 hours and stained with propidium iodide. B. Quantitation of the percentage of cell lines in the different phases from the flow cytometry analysis, n = 20000, **p < 0.005, ***p < 0.0005. C. Extracts of T98G cells were subject to Western blotting with phosphorylated Cdc Y15, vinculin, Cyclin A2, actin, phosphorylated H3 S10, γH2AX and H3 antibodies. D. Flow cytometry analysis of T98G cells expressing shHDAC8 (TRCN0000350469) and stained with propidium iodide. E. Extracts of U87 cells treated with PCI34051 were subject to Western blotting with PARP and actin antibodies.