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. 2019 Nov 21;10:5284. doi: 10.1038/s41467-019-13172-8

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Fig. 4 — NAD⁺ replenishment normalizes the transcriptomic profiles of WRN. The wrn-1(gk99) and the N2 worms were treated with NR (1 mM) from the L4 stage, followed by changing to fresh drug plate on adult Day4. The worms were collected on adult D7 for transcriptomic analysis. a Principal component analysis (PCA) revealed separation between N2 (veh) and wrn-1(gk99) (veh), while NR treatment led to a normalization of the wrn-1(gk99) transcriptomic profile closer to the N2 (veh). b Venn diagram of transcriptomic results showing that NR induced changes of genes between [N2 (NR) vs. N2 (veh.)] vs. [wrn-1 (NR) vs. wrn-1 (veh.)]. c, d Gene-set-enrichment analysis demonstrates upregulated and downregulated signaling pathways (GO pathways) in the D7 N2 and the wrn-1(gk99) worms treated with/without NR (1 mM from L4). The GO terms were ranked on the basis of enrichment scores. The upregulated GO pathways and downregulated GO pathways summarized separated, with a whole list of changes GO terms shown in Supplementary Table 3. e Heat map data showing changes of the GO terms related to mitochondrial function among four different comparisons.