Table 1.

Specifications of the used DNA extraction kits.

	DNA extraction kit	Kit number in this study	Storage of samples	Removal of human DNA	Thermal disruption	Chemical disruption	Mechanical disruption, bead beat	Binding to column	Washing	Elution	Storage temp.
MO BIO	BiOstic Bacteremia DNA Isolation Kit	1	−80 °C	no	70 °C	yes (no proteinase k)		yes	yes	30 µL solution CB5	−80
	Microbial DNA Isolation Kit	2	−80 °C	no	4 °C	yes (no proteinase K)		yes	yes	30 µL solution MD5	−20
	PowerLyzer UltraClean Microbial DNA Isolation Kit	3	−80 °C	no	4 °C	yes (no proteinase k)		yes	yes	30 µL solution MD5	−20
	UltraClean Tissue & Cells DNA Isolation Kit (Old LOT)	4	−20 °C	no	No	yes (high salt, proteinase K)		yes	yes	30 µL solution TD3 (no salt)	−80
	UltraClean Tissue & Cells DNA Isolation Kit (New LOT)	5	−20 °C	no	No	yes (high salt, proteinase K)		yes	yes	30 µL solution TD3 (no salt)	−80
	PowerSoil DNA isolation kit	6	−80 °C	no	4 °C	yes (no proteinase k)		yes	yes	30 µL solution C6	−80
Epicentre	MasterPure yeast DNA purification kit (+PureLink Genomic DNA kit)	7	−80 °C	no	65 °C	yes (+lysozyme, no proteinase K)		yes	yes	30 µL Milipore DNase free water	−20
QIAGEN	QIAamp DNA Investigator Kit	8	2–8 °C (max 48 hours)	no	56 °C	yes		yes	yes	30 µL buffer ATE	−20
	QIAamp DNA Microbiome Kit	9	2–8 °C (max 48 hours)	yes (benzonase)	56 °C	yes		yes	yes	50 µL buffer AVE	−20
Invitrogen	PureLink Genomic DNA Kit	10	−80 °C	no	55 °C	yes (+lysozyme)		yes	yes	35 µL elution buffer	−20
	PureLink Microbiome DNA purification	11	−80 °C	no	65 °C	yes (proteinase k)		yes	yes	30 µL S6	−20
Molzym	MolYsis™ Complete5 (Ultra-deep Microbiome prep kit)	12	−20 °C	yes (MolDNase B)	37 °C − > 56 °C      − > 70 °C	yes (+2−mercaptoethanol)	no	yes	yes	40 µL deionized water (70 °C)	−20