Table 2.

DNA concentrations after extraction, success rate of libraries prepared for 16S rRNA gene sequencing and percent of human DNA in samples from the nares.

Kit number	DNA concentration (ng/µl) total kit average	DNA concentration (ng/µl) Skin samples average	DNA concentration (ng/µl) Nares samples average	DNA concentration (ng/µl) E. Coli samples average	Successful libraries (16S)	% human DNA (shotgun)
1	1.99	0.05	12.17	3.03	100% (15/15)	90.1
2	0.67	0.02	3.76	0.34	67% (8/12)	90.3
3	0.52	0.01	0.91	6.85	94% (16/17)	85.3
4	0.51	0.00	3.30	NA	100% (13/13)	91.3
5	0.65	0.00	1.13	1.89	100% (16/16)	91.0
6	0.41	0.03	4.34	0.37	100% (12/12)	91.1
7	1.26	0.02	1.72	17.28	95% (17/18)	89.4
8	1.22	0.99	6.24	NA	95% (17/18)	89.8
9	0.09	0.01	0.06	0.68	95% (17/18)	57.4
10	1.95	0.03	8.69	8.68	78% (14/18)	91.7
11	0.09	0.02	0.27	0.60	39% (7/18)	88.8
12	0.39	0.04	0.72	2.36	79% (15/19)	89.6

Success of libraries for 16S rRNA sequencing was evaluated based on 194 samples in total, 26 samples affected by loading errors were excluded. Percent of human DNA in samples were found from 12 samples from the nares which were shotgun sequenced.