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. 2019 Nov 21;9:17291. doi: 10.1038/s41598-019-53756-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vβ-only domains in CAR formats express stably and show selective pMHC binding. (a) Schematic of the constructs used in the experiments. Note that the 1^st gen Vβ-only CAR will dimerize through CD3ζ TM, which is not depicted in the schematics. (b) FACS plot of HEK293T cells transfected with NY-ESO-1 (top) and MAGE-A3 (bottom) pMHC-targeted constructs. The x-axis reflects the amount of target (red) and non-target (blue) pMHC tetramer bound to cell. The y-axis reflects the amount of the TCRβ chain on the cell surface. (c) FACS plot of Jurkat cells transfected with NY-ESO-1 (top) and MAGE-A3 (bottom) pMHC-targeted constructs. The x-axis reflects the amount of target pMHC tetramers bound to cell.