Table 2.
Read number and length distribution after ISO-Seq analysis.
| Subreads | Number | |
|---|---|---|
| Subreads number | 14341324 | |
| Average subreads length(bp) | 2903 | |
| N50(bp) | 3259 | |
| Classify | ||
| CCS | 702788 | |
| 5′-primer | 605897 | |
| 3′-primer | 653411 | |
| Poly-A | 641180 | |
| Full length | 563320 | |
| Flnc | 559454 | |
| Average flnc read length(bp) | 3338 | |
| Consensus reads | 313565 | |
| Correct | Before_correction | After_correction |
| Total_number | 313565 | 313565 |
| Mean_length(bp) | 3653 | 3698 |
| Min_length(bp) | 202 | 199 |
| Max_length(bp) | 15028 | 15233 |
| N50(bp) | 4017 | 4079 |
| N90(bp) | 2325 | 2341 |
| Cluster | Number of transcripts | Number of Genes |
| <500 bp | 311 | 169 |
| 500–1 kbp | 985 | 541 |
| 1 k–2 kbp | 29976 | 14409 |
| 2 k–3 kbp | 92431 | 48198 |
| >3 kbp | 189862 | 136676 |
| Total | 313565 | 199993 |