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. 2019 Nov 21;10(12):878. doi: 10.1038/s41419-019-2115-y

Fig. 2. Hepatic vFlip expression triggers regulated necrosis in hepatocytes.

Fig. 2

a–e Representative data derived from neonatal liver tissue of vFlipAlbCre+ (n = 5) and control littermates (n = 6). Experiments were repeated three times with similar results. a Representative stainings of H&E, TUNEL (red), cl. CASP3 (green), MLKL (red), GSDMD (red) counterstained with Hoechst (blue, nuclei) in consecutive liver cross-sections. Scale bar: 100 µm. b Relative mRNA expression of Mlkl in the liver. c Detection of hepatic MLKL and GSDMD protein level by Western Blot. GAPDH was used as loading control. d Representative images via z-stack confocal microscopy from mock, Etanercept (TNF inhibitor) and Necrostatin-1 (RIPK1 inhibitor) treated liver organoids derived from embryonic vFlipAlbCre+ and control mice. Scale bar: 100 µm. e, f Relative mRNA expression of genes involved in e pyroptosis and f lipid peroxidation in the liver of indicated mice. g Detection of GPX4 protein level by Western Blot. GAPDH was used as loading control. Gene expression levels are shown relative to Hprt. Error bars indicate ± SD, ***P < 0.001, ****P < 0.0001 by unpaired two-tailed t-test.