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. 2019 Nov 21;10(12):878. doi: 10.1038/s41419-019-2115-y

Fig. 3. Impact of hepatic vFlip expression on bile acid biosynthesis and transport.

Fig. 3

a–b Representative data derived from neonatal liver tissue of vFlipAlbCre+ and control littermates at ≤7 h and ≥30 h post birth. Experiments were repeated 3 times with similar results. a Relative mRNA expression of indicated genes. b Immunohistochemical stainings from liver cross-sections stained for Albumin (ALB, red; hepatocytes) counterstained with Hoechst (blue, nuclei). Scale bar 100 µm. c Simplified schematic overview of key elements in bile production and transport affected in vFlipAlbCre+ mice. d–e Representative data derived from neonatal liver tissue of vFlipAlbCre+ (n = 5) and control littermates (n = 6). Experiments were repeated 3 times with similar results. Relative mRNA expression of genes involved in d bile acid biosynthesis and e bile transport in the liver of indicated mice. Gene expression levels are shown relative to Hprt. Error bars indicate ± SD, **P < 0.01, ***P < 0.001, ****P < 0.0001 by unpaired two-tailed t-test, intergroup comparison was performed via one-way ANOVA analysis.