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. 2019 Nov 21;10(12):878. doi: 10.1038/s41419-019-2115-y

Fig. 4. Effect of hepatocellular necrosis on the biliary system.

Fig. 4

a–c Representative data derived from neonatal liver tissue of vFlipAlbCre+ (n = 5) and control littermates (n = 6). Experiments were repeated 3 times with similar results. a Immunohistochemical staining of cytokeratin-19 (CK19, red) counterstained with Hoechst (blue, nuclei) in liver cross-sections of control or vFlipAlbCre+ mice at early (4 h p.b.) and late (80 h p.b.) time points. Scale bar 50 µm. b Relative expression of Ck19 in liver tissue. c Representative stainings of CK19 (red), MLKL (red), GSDMD (red) counterstained with Hoechst (blue, nuclei) in consecutive liver cross-sections. Scale bar 50 µm. d Representative images of immunohistochemical stainings of cholangiocytes (CK19), Kupffer cells (F4/80), neutrophils (MPO), and dendritic cells (CD11c) in liver cross-sections of vFlipAlbCre+ and control mice. Nucleic counterstaining with Hoechst (blue). Scale bar: 250 µm. Gene expression levels are shown relative to Hprt. Error bars indicate ± SD, ***P < 0.001 by unpaired two-tailed t-test.