Fig. 5. Effect of liver necrosis and defective bile acid production on intestinal homeostasis.
a–c Representative data derived from neonatal duodenum of vFlipAlbCre+ (n = 5) and control littermates (n = 6). Experiments were repeated three times with similar results. a Representative stainings from duodenum cross-sections stained with H&E, OilRed (red, lipids) and TUNEL assay (red) counterstained with Hoechst (blue, nuclei). Scale bar 100 µm. b, c Relative mRNA expression of genes involved in b lipid peroxidation and c pyroptosis in the liver of indicated mice. d Simplified schematic overview of vFLIP induced regulated necrosis in the liver. vFLIP: viral Fas-associated death domain-like interleukin-1β-converting enzyme-inhibitory protein, TNF: Tumor necrosis factor, TNFR: TNF receptor, RIPK1: Receptor-interacting protein kinase 1, MLKL: Mixed lineage kinase domain-like pseudokinase. Gene expression levels are shown relative to Hprt. Error bars indicate ± SD, **P < 0.01, ***P < 0.001 by unpaired two-tailed t-test.