Figure 5.

Increased Platelet PhosphoACC in Atherosclerotic Mice

(A–C) Female SR-B1^flox/flox/ApoE^–/–(dot) and control C57BL6 (triangle) mice received either chow diet for 24 weeks (black symbols) or 12 weeks chow diet followed by 12 weeks Western diet (red symbols) before sacrifice. Atherosclerotic burden was evaluated by (A, B) Oil-Red-O staining of the aortic root and (A, C) platelet phosphoACC by Western blot. (A) The top 2 panels show representative pictures of the aortic root (top) and of Oil-Red-O staining after cross section of the aortic root. Scale bar = 1 mm (bottom). The bottom 2 panels show representative Western blot of platelet phosphoACC. Gelsolin was used as loading control. Quantifications of (B) Oil-Red-O staining and (C) phosphoACC are shown. Data are represented as (B) medians with interquartile range or (C) boxplot. Single asterisk denotes statistical difference between C57BL6 and SR-B1^flox/flox/ApoE^–/–. Double asterisk denotes statistical differences between SR-B1^flox/flox/ApoE^–/– under high-fat diet compared with all other groups. Abbreviations as in Figures 1 and 3.