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. 2019 Nov 21;9:17251. doi: 10.1038/s41598-019-53847-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DNA-damage responses, osmotic stress and OMVs associated genes affected by PF. (A) Heat map of the differentially expressed genes associated with the DNA-damage response in A. baumannii strain A118 under 4% PF exposition. (B) Phenotype microarray heat map of strain A118 induced with or without PF in various sources of osmolytes. (C) Heat map of the differentially expressed genes associated with osmotic stress response. (D) Heat map of the differentially expressed genes associated with the OMVs biogenesis in A. baumannii strain A118 under 4% PF exposition. (E) SDS-PAGE (lanes 1 and 2) and Western blot analysis (lanes 3 and 4) of proteins of OMVs from A118. For the western blot analysis, the samples were immunoblotted with a rabbit anti-AbOmpA immune serum. Arrows indicate AbOmpA. Both SDS-PAGE and western blot were cropped to only observe content from strain A118. Brightness and contrast were adjusted to observe less visible bands. The full-length blots/gels are presented in Supplementary Fig. S5.