Table 1.
PCR primer pair sequences for selected genes.
| Gene | Accession number | Forward primer | Reverse primer | RT-PCR | qRT-PCR | ChIP | PCR product (bp) |
|---|---|---|---|---|---|---|---|
| EVA1A | NM_001135032.1 | ATGCCCCTTTTCTAGCCAGG | CAGTCGCGTTTCTCCGATCT | x | x | 153 | |
| EVA1A-AS | NR_110281.1 | CCTGCATCACTGCATTTCCG | TGCGAAAGAGTGGCACACAG | x | x | 145 | |
| GAPDH | NM_002046 | TGTTGCCATCAATGACCCCTT | CTCCACGACGTACTCAGCG | x | x | 202 | |
| ID1 | NM_002165.3 | CTCTGCACACCTACTAGTCACC | GTCACGTTTGGTGCTTCTCAGATTTC | x | 117 | ||
| Max | NM_002382 | CAATCTGCGGCTGACAAACG | GCACTTGACCTCGCCTTCT | x | 272 | ||
| Mxi1 | NM_130439 | GCGCCTTTGTTTAGAACGCTT | AATGCTGTCCATTCGTATTCGT | x | 235 | ||
| Myc | NM_002467.4 | CATGAGGAGACACCGCCCACCACCAG | TGCGTAGTTGTGCTGATGTGTGGAGACGTG | x | 210 | ||
| SPC25 | NM_020675 | TCATCTTGAGGGCCTAGCAG | CATTGTGCACATGTACCCTAAAAC | x | 221 | ||
| THOC5 | NM_001002878 | GAGACCCTCACCAGCAAACAC | CGAATGGCATAAACAGGTACTCC | x | 213 | ||
| F1-B2 | TTTGTCGTGTCCGCCCCTCAG | TGTCAGGAGCAGAGAAGTTTCTG | x | 210 | |||
| F2-B1 | CTGGACATAGTGGCATATAACTGTAG | GATGATGAACTCAGTTTCAGATATGTTG | x | 242 | |||
| F3-B2 | GTATGACCCTGGGAGACTGG | TGTCAGGAGCAGAGAAGTTTCTG | x | x | 329 | ||
| F4-B3 | ATGCCCCTTTTCTAGCCAGG | CAGTCGCGTTTCTCCGATCT | x | x | 153 | ||
| EVA1A promoter | GCCGTTCAGGTTTCGCGTCTT | CAAGCCCCGCAGAAGCTGCA | x | 176 | |||
| EVA1A-AS promoter | CTCACTCTCCAATCGGAACCTCTA | CTCATGAGAACTCACCCACTATTAG | x | 193 |
EVA1A: eva-1 homolog A, regulator of programmed cell death; EVA1A-AS: eva-1 antisense RNA; GAPDH: glyceraldehyde-3 phosphate dehydrogenase; ID1: inhibitor of DNA binding 1, HLH protein; Max: MYC associated factor X; Mxi1: MAX-interacting protein 1; Myc: v-myc avian myelocytomatosis viral oncogene homolog; SPC25: NDC80 kinetochore complex component; THOC5: THO complex subunit 5 homolog.