Figure 3.

Verteporfin Improves Pressure Overload–Induced Cardiac Hypertrophy in the Presence of HFD

(A) Schematic representation of the experimental protocol. Mice were fed with either ND or HFD for 8 weeks. Four weeks after the start of ND or HFD, mice were subjected to either sham operation or TAC in the presence or absence of verteporfin for 4 weeks. (B) Kaplan-Meier survival curves for HFD-fed mice after TAC in the presence or absence of verteporfin. (C) %FS and LVEDD evaluated with echocardiography 4 weeks after TAC, with or without verteporfin treatment (n = 6, each). (D) Left ventricular weight to tibial length (LV W/ TL) ratio (n = 6, each). (E) Lung weight to tibial length (Lung W/ TL) ratio (n = 6, each). (F) Representative gel pictures of YAP, TEAD1, OSM, MYH7, ACTA2, RUNX1, and α-tubulin in the heart (n = 6, each). All results are mean ± SEM. *p < 0.05, **p < 0.01 by analysis of variance. i.p. = intraperitoneal; N.S. = not significant; Op = operation; post op = postoperative; other abbreviations as in Figures 1 and 2.