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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Cerebellum. 2019 Dec;18(6):1036–1063. doi: 10.1007/s12311-019-01037-1

Fig. 5:

Fig. 5:

a. Examples of the current evoked from Drosophila Shab when depolarized from −133mV to between −93mV and −3mV in flies expressing only native Shab (left panel), native Shab with the human Kv9.2 (middle panel), or native Shab with hKv9.2-D379E (right panel). The non-inactivating Shab becomes inactivating in the presence of either hKv9.2 subunit. b. Diagram of the voltage step protocol used. c. The I-V relationships for Shab determined from the three given genotypes. The peak current (left panel, n = 3) shows that expression of either Kv9.2 subunit causes a shift in activation to more negative voltages. The sustained current (middle panel, n = 3) shows that, at similar voltages, flies expressing either hKv9.2 subunit show reduced Shab current after 200ms of depolarization. When the sustained current after 200ms of depolarization to −3mV is expressed as a percentage of the peak current (right panel, n = 3) the flies expressing only native Shab show high percentages, indicating very low levels of inactivation. When tested by two-way ANOVA, flies expressing either the wild-type hKv9.2 subunit (p < 0.001) or the ET mutant hKv9.2-D379E subunit (p < 0.001) were significantly different to flies expressing only native Shab. The mutant hKv9.2-D379E subunit shows much higher levels of inactivation than the wild-type hKv9.2 subunit (p=0.0462).