Clusters of mCHERRY-positive cell bodies confirm findings observed with tdTOM. Expression of mCHERRY-positive cell bodies in combination with (A–G) TH and (H–N) DAT immunofluorescence in the (A, H) midbrain: VTA and SNc (B, I) the arcuate nucleus and (C, J) the PMH (D, K) the lateral septum (E, L) the LHb (F, M) the BLP and (G, N) the RMN of mCherryTRAPDAT-Cre mice (white arrows showing mCHERRY-positive cell bodies, green arrows indicating TH-positive cells, and yellow arrows illustrating examples of co-localization; scale bar: 50 μm). See also Extended Data Figure 3-1. Arc, arcuate nucleus; CPu, caudate putamen; D3V, dorsal third ventricle; LS, lateral septum; LV, lateral ventricle; MHb, medial habenula; 3V, third ventricle Arc, Arcuate nucleus; CPu, Caudate putamen; D3V, Dorsal 3rd ventricle; LHb, Lateral habenula; LS, Lateral septum; LV, Lateral ventricle; BLP, Posterior part of basolateral amygdala; MHb, Medial habenula; PMH, Premammillary nucleus; RMN; Retromammillary; SNc, Substantia nigra pars compacta; VTA: Ventral tegmental area; 3V, Third ventricle.