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. 2019 Oct 23;6(5):ENEURO.0193-19.2019. doi: 10.1523/ENEURO.0193-19.2019

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Copyright © 2019 Kuljis et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 7. — The distribution of PV, SST, and VIP inputs across L2/3 Pyr neurons. A, PV-input assigned synapses for an example L2/3 Pyr neuron. Small light-green spheres are un-assigned FAPpost puncta; large colored spheres are input-assigned FAPpost puncta. See Extended Data Figure 7-1 for images of all input-analyzed Pyr neurons. B, Mean density of PV-assigned FAPpost contacts across dendritic branch orders. Left, Bar is mean ± SEM of all cells. Right, Individual cell values, plotted as connected lines. All data shown, statistical comparisons performed on balanced data. PV-assigned puncta density was greater for the 1° apical dendrite. RM ANOVA_PV-Input: F_(7,28) = 6.7, p = 0.002; n = 5 cells, N = 3 animals. *, Tukey post hoc pairwise comparison test, p < 0.05. C, As in A, but for SST. See Extended Data Figure 7-2 for images of all input-analyzed Pyr neurons. D, SST-assigned puncta density was not statistically significantly different across branch orders. RM ANOVA_SST-Input: F_(7,28) = 0.63, p = 0.7; n = 5 cells, N = 3 animals. E, As in A, but for VIP. Scale bar = 20 μm. See Extended Data Figure 7-3 for images of all input-analyzed Pyr neurons. F, VIP-assigned puncta density was greater for the 1° apical dendrite. RM ANOVA_VIP-Input: F_(7,42) = 3.8, p = 0.003; n = 7 cells, N = 3 animals. *, Tukey post hoc pairwise comparison test, p < 0.05. G, Inhibitory innervation of Pyr neurons, expressed as a percentage of the total number of detected synapses, for each input source. All dendritic compartments pooled for somatic and dendritic comparison. Two-way RM ANOVA_Input: F_(2,24) = 45, p < 0.001. All * show Tukey post hoc pairwise comparison test, p < 0.05. H, Pie-chart showing average proportion of input-assigned FAPpost contacts versus total detected synapses, binned as perisomatic (soma and 1° apical) or higher-order dendritic compartments (apical 3° + 4° and basal 3° + 4°). A greater proportion of PV-inputs were found on soma and 1° apical dendrite than for SST- or VIP-inputs. At higher-order apical branches, the proportion of SST-input (10.5 ± 1.9%) was similar to PV-input (16.2 ± 1.9%), but significantly greater than VIP-inputs (1.4 ± 1.9%). For higher-order basal branches, all input sources were significantly different (PV = 17.3 ± 1.4%, n = 7 cells, N = 4 animals; SST = 10.2 ± 1.4%, n = 7 cells, N = 4 animals; VIP = 2.3 ± 1.4%, n = 7 cells, N = 2 animals). Two-way RM ANOVA_Binned-Input: F_(2,18) = 47.3, p < 0.0001.