Figure 8.
IPA blocks the effect of PA on Sec18 degradation by thrombin. Full-length Sec18 was incubated with 100 μm C8-PA alone or 100 μm IPA followed by 100 μm C8-PA, after which the samples were incubated with thrombin for 30 min. Samples were mixed with SDS loading buffer, resolved by SDS-PAGE, and visualized with One-Step Blue® Protein Gel Stain. Quantitation was determined for the relative amounts of uncleaved protein. n = 3 for data shown. Mr indicates relative molecular mass markers. **, p < 0.01; ****, p < 0.0001. Major degradation products are labeled as p1, p2, and p3.