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The sequential depolymerization of RFOs by Aga and GtfA. A, the substrates sucrose, verbascose, and raffinose were incubated with Aga and/or GtfA for 18 h prior to separation by TLC. Standards and substrates are indicated below each spot. B, schematic depiction of the sequential breakdown of verbascose and raffinose by pneumococcal GHs. GHs are indicated in bold next to the arrow for the reaction they catalyze.
