Table 2.

Kinetic constants for Aga against α-galactoside substrates

The data shown are the means of three replicates.

Substrate	Km ± S.E.	kcat ± S.E.	kcat/Km ± S.E.
	mm	s−1	s−1 mm−1
Melibiose Gal-α-(1→6)-Glc	4.9 ± 0.4	162.0 ± 4.6	33.1 ± 2.9
α-(1→3)-Galactobiose Gal-α-(1→3)-Gal	2.2 ± 0.2	76.2 ± 2.3	34.6 ± 3.1
Raffinose Gal-α-(1→6)-Glc-α-(1→2)-Fru	5.8 ± 0.3	81.1 ± 1.3	14.0 ± 0.7
Stachyose Gal-α-(1→6)-Gal-α-(1→6)-Glc-α-(1→2)-Fru	11.0 ± 0.9	155.0 ± 4.3	14.1 ± 1.2