Table 2.
Construct | Velocity (nm/s) |
EC50 (μm) (n = 3) c | ||||
---|---|---|---|---|---|---|
Buffer (n = 120)a | DMSO (n = 90)b | OM (n = 90) b | DMSO (n = 90)c | OM (n = 90) c | ||
WT | 1547 ± 19 | 1771 ± 46 | 8.0 ± 0.2d,e | 1824 ± 26 | 9.9 ± 0.3d,e | 0.074 ± 0.002 |
F764L | 1300 ± 17d,f | 1539 ± 24d,f | 29.4 ± 0.8d,e,f | 1733 ± 31f,g | 44 ± 1.0d,e,f | 0.246 ± 0.008d,f |
a Four protein preparations at 0.24–0.4 μm density.
b Three protein preparations at 0.24 μm density with 20 mm KCl.
c Three protein preparations at 0.4 μm density with 100 mm KCl.
d p < 0.001.
e 10 μm OM condition compared to 0.1% DMSO.
f F764L compared to WT condition.
g p < 0.05.