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. 2019 Oct 9;294(46):17383–17394. doi: 10.1074/jbc.RA119.009589

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© 2019 Kwok et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — SYNPO-2A binding induces changes in the KWW_TD spectrum. A, schematic representation of the SYNPO-2A construct. The residues between the two PPXY motifs were replaced with two non-native alanine residues (underlined). B, ITC measurements for the KWW_TD–SYNPO-2A interaction shows a binding affinity (K_d) of 0.1 μm. C, overlay of ¹H-¹⁵N HSQC spectra of unbound (black) and SYNPO-2A–bound KWW_TD (red) at a KWW_TD:SYNPO-2A molar ratio of 1:1. Significant chemical shift dispersion in the SYNPO-2A–bound spectrum is indicative of binding-induced folding of the WW2 domain.