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. 2019 Oct 2;294(46):17543–17554. doi: 10.1074/jbc.RA119.010846

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© 2019 Moyet et al.

Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license.

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Figure 8. — ceQORH lacking its CaM-binding domain is targeted to chloroplasts in epidermal cells. Confocal microscopy was performed on plants stably expressing the following: A, GFP (GFP alone as a negative control of plastid localization; bar, 8 μm). B, TP-GFP (transit peptide of the small subunit of RuBisCO fused to GFP as a positive control of plastid localization; bar, 8 μm). C, ceQORH-GFP (plant ceQORH fused to GFP; bar, 10 μm). D, Mut2-ceQORH-GFP (ceQORH lacking its CaM-binding domain; bar, 5 μm). GFP, GFP fluorescence. Chlorophyll, chlorophyll autofluorescence. Overlay, overlay of all three channels.