Table 2.
Conservation of putative residues for 30S binding
NC, not conserved within the larger groups of sequences indicated.
| Residue (in RmtC) | Conservation (residue) |
Tested in | References | ||
|---|---|---|---|---|---|
| All m7G1405a | Intrinsic | Acquired | |||
| % | |||||
| Lys20 | 88 (Lys/Arg) | 100 (Arg) | 91 (Lys) | RmtC | This work |
| Arg50 | 100 (Arg/Lys) | 100 (Lys) | 100 (Arg/Lys) | Sgm | Ref. 19 |
| His54 | 96 (His) | 100 (His) | 100 (His) | RmtB | Ref. 16 |
| Arg68b | NC | NC | NC | RmtC | This work |
| Lys72b | NC | NC | NC | RmtC | This work |
| Arg211 | 40 (Arg)/40 (Gln) | NC | 73 (Arg)/18 (Gln) | RmtB | Ref. 16 |
| Lys236 | 72 (Arg/Lys) | 100 (Lys) | 100 (Arg/Lys) | RmtC | This work |
| Arg241 | 96 (Arg/Lys) | 100 (Arg) | 100 (Arg/Lys) | Sgm, RmtB | Refs. 16, 18, and 19 |
| Met245 | 96 (Met) | 100 (Met) | 100 (Met) | RmtB | Ref. 16c |
a Including sequences from aminoglycoside-producing bacteria (Intrinsic), pathogen-acquired enzymes (Acquired) and uncharacterized homologs in the chloroflexi.
b Residues are conserved only in within the RmtC subclade (seven of eight sequences).
c Residues previously only tested indirectly as part of a loop deletion variant in RmtB (residues 237–246).