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. 2019 Sep 3;294(46):17654–17668. doi: 10.1074/jbc.RA119.010201

Figure 4.

Figure 4.

Quantitative high-throughput screening with orthogonal WIP1 primary assays against the NCATS Pharmaceutical Collection. (A–C), Statistics from the qHTS primary screen with the RapidFire MS assay at 5 concentrations. (A), The assay performance of the screen was robust with an average Z′-factor value of 0.73 among forty 384-well plates. (B), The average %CV for the DMSO control was 8.55. (C), Among the 2,535 compounds tested, 76 inhibited WIP1 beyond 50% (3.0% hit rate). (D–F), Statistics from the qHTS primary screen with the Rh-PBP assay at 3 concentrations. (D), The assay performance of the screen was robust with an average Z′-factor value of 0.84 among six 1,536-well plates. (E), The average %CV for the DMSO control was 4.77. (F), Among the 2,535 compounds tested, 102 inhibited WIP1 beyond 50% (4.0% hit rate).