Table 1.
RapidFire MS settings and information about precursor and product ions for each multiple reaction monitoring transition
| VEPPLpSQETFS | VEPPLSQETF*Sa | VEPPLSQETFS | |
|---|---|---|---|
| Peptide number | 3 | 4 | 5 |
| Precursor ion | 657.3 | 620.5 | 617.3 |
| MS1 resolution | Unit | Unit | Unit |
| Product ion | 1061.5, 253.2 | 611.6, 536.3 | 536.4, 253.2 |
| MS2 resolution | Wide | Wide | Wide |
| Dwell (ms) | 10 | 10 | 10 |
| Fragmentor (V) | 135 | 135 | 135 |
| Collision energy (V) | 12, 16 | 8, 10 | 10, 14 |
| Cell acceleration (V) | 5 | 5 | 5 |
| Polarity | Positive | Positive | Positive |
a F*, ring 13C6-labeled phenylalanine.