Table 5.
Direct binding of NCGC00185994 to WIP1, as measured by surface plasmon resonance, with GSK2830371 as a positive control
All data were fit to a two-state kinetic binding model. Differences in the Rmax values are attributed to the lower immobilization level of channel 1 compared with channel 6.
| Compound Name | GSK2830371 | NCGC00185994 |
|---|---|---|
| ka1 (m−1 s−1) | 2.66 × 105 | 1.70 × 104 |
| kd1 (s−1) | 7.12 × 10−2 | 5.57 × 10−1 |
| ka2 (s−1) | 1.42 × 10−2 | 6.77 × 10−3 |
| kd2 (s−1) | 7.99 × 10−4 | 1.64 × 10−2 |
| Kd (m) | 1.43 × 10−8 | 2.31 × 10−5 |
| Rmax (RU) | 15.8 | 46.7 |
| χ2 (RU2) | 1.43 × 10−1 | 3.35 × 10−1 |