Figure 4.

NK cell-derived IL-10 is dispensable during acute MCMV infection. Il10^fl/fl (Cre−) and NKp46-Cre-Il10^fl/fl (Cre+) mice were either uninfected or given 12,000 PFU or 50,000 PFU MCMV i.p. for the following analyses: (A) Body weight change of infected mice relative to their initial body weight at day 0 post-infection (n = 3; two experiments). (B) Viral titer in the spleen of Il10^fl/fl and NKp46-Cre-Il10^fl/fl mice at day 5 post-infection (n = 3; two experiments). (C) Serum levels of ALT enzyme at the indicated days post-infection (three experiments; n = 5 for D0 Cre−, n = 4 for D0 Cre+, n = 6 for D4 Cre−, n = 6 for D4 Cre+, n = 5 for D5 Cre−, n = 4 for D5 Cre+). (D) Serum levels of IL-10, IFNγ, and TNFα were measured at day 4 post-infection (n = 6; two experiments). (E) NK cells and T cells from the spleens and livers of Il10^fl/fl and NKp46-Cre-Il10^fl/fl mice at day 5 post-infection were stained for IFNγ production (n = 3; two experiments). Data represent mean + SD. ns, non-significant; *p < 0.05; **p < 0.01.