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. 2019 Nov 15;10:1441. doi: 10.3389/fpls.2019.01441

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Copyright © 2019 Ullah, Unsicker, Reichelt, Gershenzon and Hammerbacher

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Concentrations of (A) salicylic acid (SA), (B) jasmonic acid (JA), and (C) JA-isoleucine (JA-Ile) in black poplar stems increase after P. populi infection. Trees approximately 1 m in height were inoculated with P. populi after wounding and control trees were inoculated with sterile culture medium. Analysis was performed by LC-tandem mass spectrometry. Samples were harvested from separate trees at each time point. Data were analyzed using Student’s t-test (*, p ≤ 0.05; **, p < 0.01; and ***, p < 0.001; ns, non-significant). Data represented in figures are mean ± SE (n = 5). dpi, days post inoculation; DW, dry weight.