Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 21;21:123. doi: 10.1186/s13058-019-1216-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 6 — WDR5-TGFβ1 inhibition reduces cell migration and EMT gene expression in breast cancer. a Effect of WDR5 inhibitor OICR-9429 at increasing concentrations (1 μM, 5 μM, 10 μM, 20 μM, 3 days of treatment) was evaluated in MDA-MB-231 cell line. Cells were plated on transwells to test in vitro cell migration, stained with crystal violet, and quantified using ImageJ analysis. Histograms represent relative cell migration expressed as mean ± SD of a biological triplicate. Differences among groups were calculated by applying one-way ANOVA and Dunnett post test (*P < 0.05; ***P < 0.001). b TGFβ1 mRNA levels (mean ± SD of n = 3 experiments) were evaluated by RT-PCR upon OICR-9429 treatment (20 μM) in MDA-MB-231 cells. Statistical differences were calculated by applying a Student t test (*P < 0.05). c MDA-MB-231 cells were treated with OICR-9429 (20 μM) and galunisertib (10 μM—TGFβ1 inhibitor) for 3 days and cells were plated on transwells for the evaluation of in vitro cell migration. Differences among groups were evaluated by applying one-way ANOVA and Dunnett post test (***P < 0.001). d, e VIM, CDH2, SNAI1, and SNAI2 expression was evaluated by RT-PCR (d) and immunofluorescence (e) upon OICR-9429 (20 μM) and galunisertib (10 μM) treatments. Differences in mRNA levels among groups were evaluated by applying an unpaired Student t test (*P < 0.05; ***P < 0.001). For immunofluorescence, DAPI (in blue) was used for nuclei staining and tubulin (in green) for cell morphology staining. VIM, CDH2, SNAI1, and SNAI2 are labeled in red. f Effects of OICR-9429 (20 μM) on TGFβ1 mRNA levels were evaluated in one TN (MBC2) and one LB (MBC26) PDXs. Statistical differences among groups were calculated by applying a Student t test (**P < 0.01; ***P < 0.001). g Effects of OICR-9429 (20 μM) and galunisertib (10 μM) were evaluated in terms of in vitro cell migration after 3 days of treatment in MBC2 and MBC26 PDX cells (mean ± SD of n = 3 experiments). Differences among groups were evaluated by applying one-way ANOVA and Dunnett post test (**P < 0.01; ***P < 0.001). h mRNA levels of VIM, CDH2, SNAI1, and SNAI2 were evaluated by RT-PCR due to aforementioned treatments in MBC2 and MBC26 PDX cells. Statistical significances among groups were calculated by applying a Student t test (*P < 0.05; **P < 0.01; ***P < 0.001)