Table 3.
RT-qPCR primer sequences
| Gene | Forward (5′−> 3′) | Reverse (5′−> 3′) | RefSeq accession number |
|---|---|---|---|
| CD25 | cgcagaataaaaagcgggtca | acttgtttcgttgtgttccga | NM_000417.2 |
| dCas9 | ggatcgaagagggcatcaaa | gttcctggtccacgtacatatc | KR011748.1 |
| FOXP3 | gcaccttcccaaatcccagt | ggccacttgcagacacca | NM_014009.3 |
| GAPDH | tgcaccaccaactgcttagc | ggcatggactgtggtcatgag | NM_002046.7 |
| GITR | ccagtgtatcgactgtgcctcg | cacagcgttgtgggtcttgttc | NM_004195.3 |
| ICOS | cccataggatgtgcagcctttg | ggctgtgttcactgctctcatg | NM_012092 |
| IKZF2 | acactctggagagaagccgttc | ccagtgaactgcgctgcttgta | NM_016260.3 |
| IRF4 | gaacgaggagaagagcatcttcc | cgatgccttctcggaactttcc | NM_002460.4 |
| PI16 | ctggtgtgcaactatgagcctc | ggcaaatcctgagcatcttccg | NM_153370.3 |
| PTPRC | cttcagtggtcccattgtggtg | ccactttgttctcggcttccag | NM_002838.5 |
| TNFR2 | cgttctccaacacgacttcatcc | acgtgcagactgcatccatgct | NM_001066.3 |