Table I.
Primers of mtDNA-encoded complex I genes.
| Target genes | Forward sequence (5'-3') | Reverse sequence (5'-3') | Annealing temperature (˚C) | PCR product (bp) |
|---|---|---|---|---|
| ND1 | CTCAACTTAGTATTATACCC | GAGCTTAGCGCTGTGATGAG | 59 | 1,249 |
| ND2 | GTCATCTACTCTACCTAC T | GGCGGGAGAAGTAGATTGAA | 52 | 689 |
| ND3 | CACTATCTGCTTCATCCGCC | GAGCGATATACTAGTATTCC | 54 | 1065 |
| ND4 | GCGCAGTCATTCTCATAATC | TTTGTTAGGGTTAACGAGGG | 54 | 729 |
| NDL4 | TCTGGCCTATGAGTGACTAC | ACTGTGAGTGCGTTCGTTCGTAGTTTGAG | 54 | 1,415 |
| ND5 | TTTTGGTGCAACTCCAAA | GGTTGACCTGTTAGGGTGAG | 50 | 1,369 |
| ND6 | CTCCAAAGACCACATCATCGAAAC | TTCATCATGCGGAGATGTTGGATGGGGTGG | 52 | 1,334 |