Fig. 2.

Regional heterogeneity of fibrinogen extravasation based on the category of white matter (WM) lesion (WML) in CADASIL patients. Luxol fast blue stained (a, d, g, j, m), Haematoxylin & Eosin (H&E) stained (b, e, h, k, n) and Fibrinogen immunostained (c, f, i, l, o) serial sections from a control brain (a-c), and from normal appearing WM (NAWM) (d-f), “pure” WML (g-i), enlarged perivascular space (ePVS) WML (j-l) and damaged WM around a lacune (m-o) in the brain of a CADASIL patient. Graphs show quantification of fibrinogen staining in the first (p) and second (q) cohorts of CADASIL patients. Mean ± SEM; *: p < 0.05, **: p < 0.01; one-way ANOVA with Tukey’s post-hoc tests; cohort 1: n = 6 (controls), 8 (patients); cohort 2: n = 4 (controls), 4 (patients). Scale bar: 50 μm