Fig. 2.

The osteogenic differentiation of hASCs under cyclic strain (*p < 0.05, significant differences existed between these two groups). a qPCR results. The mRNA levels of RUNX (22.47 ± 0.73-fold, p = 0.011), ALP (2.57 ± 0.09-fold, p = 0.004), SPARC (2.13 ± 0.60-fold, p = 0.018), LEF1 (1.89 ± 0.45-fold, p = 0.012), and β-catenin (2.22 ± 0.51-fold, p = 0.017) were significantly increased. b Western blot results. The pictures of protein blots are shown in the left column. The protein expression levels of RUNX2 (2.59 ± 0.31-fold, p = 0.001), ALP (1.39 ± 0.09-fold, p = 0.010), SPARC (1.79 ± 0.21-fold, p = 0.005), LEF1 (1.55 ± 0.39-fold, p = 0.030), and β-catenin (1.63 ± 0.20-fold, p = 0.004) were significantly increased