Table 2.
The primer sequences used for qPCR in this study
| Gene | Accession no. | 5′-3′ | Tm (°C) |
|---|---|---|---|
| RUNX2 | NM_001015051 | F: TAGATAGTGATTGCGTTTGGCTATG | 60 |
| R: CACTAAGAAATGTTTCAAGGGTCC | 60 | ||
| ALP | NM_003064 | F: GAAAGTCCTTCAAAGCTGGAGTCT | 60 |
| R: TCTGGCACTCAGGTTTCTTGTATC | 60 | ||
| SPARC | NM_001309443 | F: TGTGATCTAAATCCACTCCTTCCA | 60 |
| R: ACAAACCATCCAAACATTTTAAACA | 60 | ||
| LEF1 | NM_001130714 | F: TGCCAAATATGAATAACGACCCA | 60 |
| R: GAGAAAAGTGCTCGTCACTGT | 60 | ||
| β-catenin | NM_001098209 | F: AAAATGGCAGTGCGTTTAG | 60 |
| R: TTTGAAGGCAGTCTGTCGTA | 60 | ||
| GAPDH | NM_001256799.2 | F: GAACGGGAAGCTCACTGG | 60 |
| R: GCCTGCTTCACCACCTTCT | 60 |