Table 1.
Primers for PCR-based assays and siRNAs used for transfection
| Target | Oligonucleotide sequence |
|---|---|
| qPCR | |
| OPNt | F: GCCGAGGTGATAGTGTGGTT |
| R: AACGGGGATGGCCTTGTATG | |
| OPNa | F: GCCGAGGTGATAGTGTGGTT |
| R: AACGGGGATGGCCTTGTATG | |
| OPNb | F: ATCTCCTAGCCCCACAGAC |
| R: AAAATCAGTGACCAGTTCATCAG | |
| OPNc | F: TGAGGAAAAGCAGAATGCTG |
| R: GTCAATGGAGTCCTGGCTGT | |
| β-actin | F: CATTAAGGAGAAGCTGTGCT |
| R: ACTGAACCTGACCGTACAGCTCGTAGCTCTTCTCCAG | |
| GAPDH | F: GGAGCGAGATCCCTCCAAAAT |
| R: GGCTGTTGTCATACTTCTCATGG | |
| PCR | |
| OPN | F: AGCAGAATCTCCTAGCCCCA |
| R: ACGGCTGTCCCAATCAGAAG | |
| E-cadherin | F: TGCCCAGAAAATGAAAAAGG |
| R: GTGTATGTGGCAATGCGTTC | |
| N-cadherin | F: GACAATGCCCCTCAAGTGTT |
| R: CCATTAAGCCGAGTGATGGT | |
| Vimentin | F: CTGCAGGACTCGGTGGACTT |
| R: GAAGCGGTCATTCAGCTCCT | |
| Snail | F: TACAGCGAGCTGCAGGACTCTAAT |
| R: AGGACAGAGTCCCAGATGAGCATT | |
| Slug | F: TGATGAAGAGGAAAGACTACAG |
| R: GCTCACATATTCCTTGTCACAG | |
| Twist | F: GCCGACGACAGCCTGAGCAA |
| R: CGCCACAGCCCGCAGACTTC | |
| RUNX2 | F: ACGGCAGCGGACAGCAGA |
| R: TGCGGATAGCAACACAGTTCT | |
| GAPDH | F: GGCTGCTTTTAACTCTGGTA |
| R: GACTGTGGTCATGAGTCCTT | |
| siRNA | |
| scramble | UUCUCCGAACGUGUCACGUTT |
| SRSF1 | CCAACAAGATAGAGTATAATT |
| TGAAGCAGGTGATGTATGTTT | |
| RUNX2 | CUCUGCACCAAGUCCUUUUTT |
| GGUUCAACGAUCUGAGAUUTT | |