Fig. 3.

Relevant genetic features of ST398 LA-MRSA from swine and farm workers. a IS256 insertion in the SAPIG2195 gene, detected in all t011 MRSA isolates from farm ID 05CS. The insertion position (nt 385) is relative to the SAPIG2195 coding sequence of S. aureus WCH-SK2 (CP031537). b, c Rep-MP3 PCR and RAPD fingerprints of MRSA isolates from workers and swine belonging to farm ID 01CZ and 32RC, respectively. The dendrogram was generated with BioNumerics using UPGMA and the Dice coefficient. S. aureus ATCC 43300 was included as outgroup for analysis. Squares denote the source of MRSA; red, human; green, swine. d Pairwise ANI comparison matrix of MRSA genomes, calculated with JSpecies. ANI values > 99.90% are in grey. e Phylogenetic tree based on SNPs analysis obtained by CSI phylogeny and visualized with MEGA. LA-MRSA strain S0385 was used as reference. Branch lengths are the number of substitutions per site