Fig. 9.

LncRNA-LET inhibits tumor growth in vivo. 786-O cell xenograft tumors stably transfected with lncRNA-LET or EV vectors, as well as 769-P cell xenograft tumors stably transfected with lncRNA-LET shRNA or shRNA Ctrl vectors were obtained. The size of the tumor was then recorded every 3 days for 21 days. Finally, the mice were sacrificed, and tumor tissues were (a) took pictures. b The volume of tumor tissues were measured by caliper and then calculated. c Cell apoptosis and necrosis from tumor tissues was analyzed using HE staining. Scale bars, 100 μm. d Cell apoptosis in tumor tissues was detected through TUNEL staining. Scale bars, 50 μm. e A representative Ki67 immunofluorescence in tumor tissues was explored. Scale bars, 50 μm. n = 6, ^##p < 0.01. ccRCC clear cell renal cell carcinoma, lncRNA-LET lncRNA-LET overexpression, EV lncRNA-LET overexpression matched control, lncRNA-LET shRNA lncRNA-LET knockdown, shRNA Ctrl lncRNA-LET knockdown control