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. 2019 Nov 22;20(Suppl 9):406. doi: 10.1186/s12859-019-2886-2

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — a The diagram represents the intersection between the set of genes with human-specific features and the set of genes that undergo differential expression upon activation of iPSC-derived neurons with KCl for 3 h. b The 12 human-specific differentially expressed genes and their mechanism of origin. c Gene expression was investigated by RT-qPCR using RNA from the same samples of active and inactive iPSC-derived neurons. Fold-change values were calculated relative to GAPDH expression. Statistical significance was performed using unpaired t-test (*p-value < 0.05; ***p value < 0.001)