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. 2019 Nov 21;20:51. doi: 10.1186/s12860-019-0235-y

Table 1.

Primers sequences for RT-PCR and Q-PCR analysis

Gene Primer Sequence Length (bp) Application
p53

F: ACAGCGTGGTGGTACCGTAT

R: GGAGCTGTTGCACATGTACT

83 Q-PCR
p21waf1

F: TCTTGCACTCTGGTGTCTCA

R: GGGCTTTCTCTTGCAGAAG

147 Q-PCR
p16INK4A

F: TGCAGATAGACTAGCCAGGGC

R: CTCGCAGTTCGAATCTGCAC

184 Q-PCR
GAPDH

F: GCAAGAGAGAGGCCCTCAG

R: TGTGAGGGAGATGCTCAGTG

74 Q-PCR
rTERT

F: GCTCCGGTTACACAGCAGCCCCTGGCA

R: GGTCCAGAGCACGCACACGCAGCACGA

752 RT-PCR
TR

F: CTCCGCCCGCTGTTTTTCTCGCTGACT

R: GCCACCGAACTCAGGGACCAGTTCCGT

300 RT-PCR
CD73

F: TCCCGCGGCTGCTACGGCACCCAAGTG

R: ACCTTGGTGAAGAGCCGGGCCACGCCG

204 RT-PCR
CD90

F: CCTGACCCGAGAGAAGAA

R: TGAAGTTGGCTAGAGTAAGGA

125 RT-PCR
CD105

F: CGGTCTCCAGCTGCGGTGGTGGGCTCC

R: CACTGCCACCACGGGCTCCCGCTTGCT

896 RT-PCR
CD34

F: GTCACACTGCCTACTACTTC

R:TCCTCGGATTCCTGAACAT

210 RT-PCR
CD45

F: AGCAATACCAGTTCCTCTATGA

R: TCCGTCCACTTCGTTATGA

113 RT-PCR
CD106

F: TCCACACTGACGCTGAGCCCTGTGGGTG

R: CTCCGGCATCCTGCAGCTGTGCCTTGCG

898 RT-PCR
CD146

F: GCAGCGCCACGGGTGTGCCAGGAGAGG

R: CCCCACTGTGGTGCTTCTGGGCGGGCT

900 RT-PCR
Nanog

F: GGGGATTCCTCGCCGATGCCTGCCGTT

R: GGGATACTCCACCGGCGCTGAGCCCTT

477 RT-PCR
Oct4

F: GCCCACCTTCCCCATGGCTGGACACCT

R: GCAGGGCCTCGAAGCGGCAGATGGTTG

563 RT-PCR
Slug

F: CACTCCCCTCTGCCCAGCGGCCTTTCT

R: GGCATGGGGGTCTGAAAGCTTGGGCTG

171 RT-PCR
Snail

F: GCGAGCTGCAGGACGCGTGTGTGGAGT

R: CGGCAAAGGCACGGTTGCAGTGGGAGC

597 RT-PCR
β-actin

F: TGAAGATCAAGATCATTGCTCCTCC

R: CTAGAAGCATTTGCGGTGGACGATG

155 RT-PCR
Osterix

F: GCTTTTCTGTGGCAAGAGGTTC

R: CTGATGTTTGCTCAAGTGGTCG

136 Q-PCR
OCN

F: AAGCCCAGCGACTCTGAGTCT

R: CCGGAGTCTATTCACCACCTTACT

75 Q-PCR
Adiponectin

F: GAATCATTATGACGGCAGCAC

R: CTTGGAGCCAGACTTGGTCTC

224 Q-PCR
PPARγ

F: GGAAGCCCTTTGGTGACTTTATGG

R: GCAGCAGGTTGTCTTGGATGTC

174 Q-PCR
β-actin

F: TGAAGATCAAGATCATTGCTCCTCC

R:CTAGAAGCATTTGCGGTGGACGATG

108 Q-PCR