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. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Lung Cancer. 2019 Feb 25;130:169–178. doi: 10.1016/j.lungcan.2019.02.027

Figure 2. siRNA-mediated inhibition of IKKβ expression decreases HUVEC migration, invasion and tube formation in vitro.

Figure 2.

A549 cells were transfected with a non-targeting control siRNA (siCtrl) or with siRNA smartpools targeting KRAS (siKRAS) or IKKβ (siIKKβ) and conditioned medium was collected as described in methods. (a) Transwell migration assay of HUVEC cells was performed using conditioned medium from A549-transfected cells as chemoattractant as indicated. Conditioned medium was supplemented with 5 ng/ml IL-8 and 5ng/ml VEGF as indicated (lower panel). Images shown are representative of three independent experiments. (b) Transwell matrigel invasion assay of HUVEC cells was performed using conditioned medium from A549-transfected cells as chemoattractant as indicated. Images shown are representative of three independent experiments. (c) Endothelial cell tube formation assay. HUVEC cells were plated onto Matrigel in the presence of serum-free conditioned medium from A549- or H358-transfected cells as indicated. Images shown are representative of three independent experiments. In all cases, bar graphs represent average ± 1s.d. Statistical significance was measured by one-way ANOVA followed by Bonferroni’s post-test (**p<0.01, ***p<0.001) by comparing siCtrl-transfected groups with siKRAS- and/or siIKKβ-transfected groups. Groups being compared are indicated by horizontal bars.