FIGURE 2.

Effect of IS on immune phenotypes in spleens and kidney allograft. Kidneys from BALB/c (CD45.1) were transplanted into B6 recipients (CD45.2) with immunosuppression (IS) (w/IS) or without IS (wo/IS) (n = 4-6/group). Cells isolated from spleens and renal grafts were analyzed by flow cytometry. Frequencies of recipients’ T cells (gated on CD45.2⁺CD3⁺ cells) and myeloid cells (gated on CD45.2⁺CD11b⁺ cells), including dendritic cells (DCs) (CD11c⁺MHCII⁺ DCs), Mac (CD11b⁺F4/80⁺ macrophages), Mono (CD11b⁺Ly6C⁺ monocytes), and Granu (Ly6G⁺ granulocytes) are calculated based on total number of live cells and normalized by tissue weight (mg). Data in bar graphs are expressed as mean value with SEM. (*P ≤.05 or ^**P <.01, wo/IS vs w/IS). A, Representative dot-plot demonstrating percentages of recipients’ T cells and myeloid cells and absolute number of subtypes in spleens at POD2. B, Representative dot-plot demonstrating percentages of recipients (CD45.2⁺) vs donor cells (CD45.1⁺) and their absolute numbers in the kidney allografts at POD2. C, Representative dot-plot demonstrating percentages of recipients’ T cells and myeloid cells and absolute number of subtypes (above mentioned) in the kidney allografts at POD2