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. Author manuscript; available in PMC: 2019 Nov 22.
Published in final edited form as: Am J Transplant. 2019 May 14;19(9):2421–2433. doi: 10.1111/ajt.15376

FIGURE 5.

FIGURE 5

Quantification of cellular histone posttranslational modifications after transplant. Kidney tissues samples were collected from D+-to-R− kidney allografts (n = 3/group/time) treated with immunosuppression (IS) (w/IS; red) or without IS (wo/IS; black) at 3, 24, and 48 hours posttransplant. Contralateral kidneys from the donor were controls (0 hours). Histones were extracted and analyzed with liquid chromatography-tandem mass spectrometry. The relative abundance of each histone modification is determined by calculating the peptide peak area for a peptide of interest and dividing by the sum of the peak areas for all peptides with that sequence, based on the mean of 3 technical replicates with error bars representing the standard deviation. Data shown as percent of given modification in total pool of given peptide that was quantified. *P <.05; **P <.01 compared with 0 hours controls