Proteome profiling on kidney allografts and plasma protein analysis. Kidney tissues or plasma samples were collected from D+-to-R− kidney allografts treated with immunosuppression (IS) (w/IS) or without IS (wo/IS) at the indicated endpoints. The contralateral latent donor kidney (con) was recovered at the time of the transplant (postoperative [POD]0). Pathways with –log P value >1.3 (dashed line) are statistically significant. A, Mass spectometry analysis for proteins extracted from the kidney tissues at 3, 24, and 48 hours posttransplant (n = 3/group/time), followed by ingenuity pathway analysis. B, Multiplex proteomic analysis on plasma samples collected from D+/R− kidney transplants at 48 hours posttransplant (n = 6-8/group) and naive B6 (con). NS, not significant