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. 2019 May 29;55(53):7671–7674. doi: 10.1039/c9cc02655j

Scheme 2. Bioconjugation of VNAR E4 6 with PD 5 and subsequent “click” reaction with Alexafluor®-488-N3. Reagents and conditions: (vi) TCEP·HCl (10 eq.), PD 5 (20 eq.), phosphate buffer pH 7.4, 21 °C, 16 h. (vii) Alexafluor®-488-N3, phosphate buffer pH 7.4, 21 °C, 5 h.

Scheme 2